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Punnett Square Calculator

Calculate offspring genotype and phenotype ratios from a genetic cross.

Results

Dominant Phenotype %75%
Recessive Phenotype %25%
Genotype Ratio1:2:1 (AA:Aa:aa)
Carrier % (Heterozygous)50%
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What is Punnett Square?

The Punnett Square Calculator predicts the genotype and phenotype ratios of offspring from a genetic cross between two parents. Named after British geneticist Reginald Punnett, this tool is fundamental to Mendelian genetics and is taught in every biology class worldwide.

A Punnett Square is a grid that shows all possible combinations of alleles that offspring can inherit from their parents. For a monohybrid cross (one gene, two alleles), the classic Mendel ratios are:

- Homozygous dominant (AA) × Homozygous dominant (AA) = all AA - Heterozygous (Aa) × Heterozygous (Aa) = 1 AA : 2 Aa : 1 aa (3:1 phenotype ratio) - Heterozygous (Aa) × Homozygous recessive (aa) = 1 Aa : 1 aa (1:1 ratio, test cross)

In this calculator, '2 dominant alleles' = AA (homozygous dominant), '1 dominant allele' = Aa (heterozygous/carrier), '0 dominant alleles' = aa (homozygous recessive). The dominant phenotype is expressed when at least one dominant allele is present.

Formula

Punnett Square for Monohybrid Cross:

Parent genotypes → 4 possible offspring combinations

Aa × Aa cross: A a A | AA | Aa | a | Aa | aa |

Result: 1 AA : 2 Aa : 1 aa Phenotype: 75% Dominant : 25% Recessive (3:1 ratio) Carriers (Aa): 50%

All possible crosses: AA × AA = 100% AA (all dominant) AA × Aa = 50% AA, 50% Aa (all dominant, 50% carriers) AA × aa = 100% Aa (all dominant, all carriers) Aa × Aa = 25% AA, 50% Aa, 25% aa (3:1 ratio) Aa × aa = 50% Aa, 50% aa (1:1 ratio, test cross) aa × aa = 100% aa (all recessive)

How to use this Punnett Square Calculator?

1. Enter the number of dominant alleles for Parent 1: 2=AA, 1=Aa, 0=aa. 2. Same for Parent 2. 3. See the offspring phenotype percentages, genotype ratio, and carrier percentage.

Examples: Eye color (brown=dominant, blue=recessive), tongue rolling, widow's peak, attached earlobes. Note: most real traits are polygenic (controlled by many genes), so Punnett Squares are most accurate for single-gene traits.

Frequently asked questions

What is the 3:1 ratio?
When two heterozygous parents (Aa × Aa) are crossed, 75% of offspring show the dominant phenotype and 25% show recessive — a 3:1 ratio. This is Mendel's famous Law of Segregation.
What is a test cross?
A test cross is breeding an organism showing the dominant phenotype with a homozygous recessive (aa) to determine if the dominant organism is AA or Aa. If any recessive offspring appear, the parent is Aa.
Can Punnett Squares predict human traits?
For simple Mendelian traits (single gene), yes — like blood type, sickle cell carrier status, and cystic fibrosis. But most visible traits (height, skin color, intelligence) are polygenic and environmental, so Punnett Squares don't apply simply.
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